| Biological Information | |
|---|---|
| Background Information: | The SPRINTer™ eXpress IKZF1 Protein Turnover Biosensor Assay (K562) provides an easy-to-use cell based assay to monitor drug-induced protein turnover of a specific endogenous target. Genome engineering or editing techniques, such as CRISPR/Cas9, are used to introduce a small tag into the endogenous locus of interest. This allows for the quantitation of changes in target protein levels when cells are treated with therapeutic agents such as targeted protein degraders. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | Signaling Pathway |
| Accession Number: | Q13422 |
| Target Name: | IKZF1 |
| Target Aliases: | CVID13, Hs.54452, IK1, IKAROS, lyphoid transcription factor LyF-1, LYF1, protein phosphatase 1, regulatory subunit 92 PPP1R92, PRO0758, zinc finger protein, subfamily 1A, ZNFN1A1, IKAROS family zinc finger 1 |
| Target Species: | Human |
| Cell Background: | K562 |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Targeted Protein Degradation |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Targeted Protein Degradation (TPD) |
| Detection Method: | Chemiluminescence |
| Clinical Relevance | |
| Therapeutic Area: | Oncology/Immuno-Oncology |
| Additional Information | |
| Brand: | SPRINTer™ |
SPRINTer™ eXpress IKZF1 Protein Turnover Biosensor Assay (K562)
The SPRINTer™ eXpress IKZF1 Protein Turnover Biosensor Assay (K562) is used for monitoring drug-induced protein turnover of IKZF1. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-451 SPRINTer_eXpress_Endogenous_Protein_Turnover_Biosensor_Asssay User_Manual_REV0
View DocumentDatasheets
DRx_DS_91-1007E042_SPRINTer_ eXpress_K562_IKZF1_Biosensor_20240130-1
View Document