GPCR Calcium Product Solutions
Measure Calcium Mobilization Using a Homogeneous, Dye-based Assay with Stable GPCR Cell Lines
Cell-based calcium assays measure intracellular calcium ion flux from the endoplasmic reticulum (ER) to the cytosol upon ligand- or therapeutic-activation or inhibition of GPCRs or calcium ion channels in a dose-dependent manner. Eurofins DiscoverX® offers traditional and native Gq-coupled GPCR cell lines, ready-to-assay frozen GPCR cells, and a Calcium No WashPLUS detection kit.
The cell lines and frozen cells are qualified for use with FLIPR® or the homogeneous fluorescent dye-based detection kit. Rank order potential leads and perform high throughput screening (HTS) calcium mobilization analysis with the Calcium No WashPLUS detection kit and obtain large assay windows and wide dynamic ranges.
- Optional Formats – Select from hundreds of stable cell lines and frozen ready-to-assay cells, as well as the accompanying Calcium No WashPLUS fluorescent detection kit for calcium mobilization analysis in response to agonist or antagonist stimulation
- Optimized Assays – Native cell lines with overexpressed naturally Gq-coupled, wild-type GPCRs optimized for use with the Calcium No WashPLUS detection kit
- Multiplexing Capabilities – Perform calcium detection and β-arrestin recruitment assays using the same cell line in the same well for multiple readouts
- Quick Analysis – Perform single-step, HTS with Calcium No WashPLUS assays in adherent or suspension cells for both GPCRs and calcium ion channels
- Calcium No WashPLUS Detection Kit – Rapidly quantify calcium flux with homogeneous, fluorescent dye-based assays qualified for use with all Gq-coupled cell lines
- Coelenterazine H – Utilize a derivative of native coelenterazine that is significantly more sensitive to calcium and provides enhanced performance in aequorin-based luminescent HTS calcium assays. Note aequorin-based luminescence assays require addition of a coelenterazine substrate like coelenterazine H.
Cell Lines and Ready-to-Assay Frozen Cells
- Calcium Gq-coupled Cell Lines – Naturally coupled, stable Gq-coupled cell lines and qualified for use with the Calcium No WashPLUS Assay, AssayComplete™ cell culture reagents, and control ligands
- ChemiSCREEN Stable Cell Lines, Parental Cell Lines, and Ready-to-Assay Frozen Cells – Cells based on our proprietary Chem-1 and related host cell backgrounds that contain an endogenous expression of Gα15. Gα15 is a promiscuous G protein to help funnel signaling to a common calcium readout, regardless of G protein coupling status. Use cell lines or frozen cells with chemiluminescent or fluorescent assays, and parental cell lines to generate your own GPCR stable cell lines. Additional second messenger signaling, e.g. cAMP, and ERK phosphorylation, for most targets can be used to perform orthogonal assay confirmation.
- ChemiBRITE Stable Cell Lines and Ready-to-Assay Frozen Cells – Cells engineered to flash with added brightness upon GPCR activation and developed to provide the greatest readout flexibility, including luminescent and fluorescent calcium flux as well as cAMP. These cells express a proprietary mutant version of clytin, a calcium activated photoprotein.
- Homogeneous single step, no wash HTS friendly assay protocol
- Detect calcium mobilization in adherent or suspension cells for either GPCRs or calcium ion channels
- Large assay windows, wide dynamic range, and improved signal and performance over FLUO-3
- Multiplex with β-arrestin recruitment assays using the same cell line and the same well for multiple readouts
- Native cell lines overexpressing naturally Gq-coupled, wild-type GPCRs
- Optimized for use with Calcium No WashPLUS assay for detection of calcium mobilization in response to an agonist stimulation
- Cell lines containing a promiscuous G protein, Gα15, to help funnel signaling to a common calcium readout, regardless of G protein coupling status
- High functional expression of receptors with the ability to assay both agonist and antagonist in a single well
- Stable cell lines for continuous culture with increased reliability and reproducibility assay results
- Perform orthogonal assay confirmation such as second messenger signaling, e.g. cAMP, and ERK phosphorylation, for most targets
- Cell lines modified to flash with added brightness upon GPCR activation
- Exceptional high signal-to-background ratio with no assay interference with auto-fluorescent compounds
- Tool box capabilities allows generation of your own stable cell lines
- Ready-to-assay GPCR frozen cells come with plating media and enough cells to run either 96- or 384-well plate assays in full or half plate formats
- Qualified for cAMP and calcium second messenger assays with results within 24 hours to accelerate your data generation
Calcium No WashPLUS Assay Principle
The calcium G protein-dependent pathway involves a heterotrimeric (α/β/γ) G-protein containing a GDP molecule (not shown here) bound to the Gα subunit, which holds the trimer together. Upon activation, GDP is exchanged for GTP, leading to the dissociation of the Gβ/Gγ dimer from Gα. Both parts remain anchored to the membrane and become free to act upon their downstream effectors and initiate unique intracellular signaling responses. The activated Gα subunit interacts with and regulates many effector molecules such phospholipase C (PLC) that can ultimately lead to the release of calcium (a second messenger) from the ER.
The Calcium No WashPLUS Detection Kit is calcium mobilization, cell-based assay which uses an esterified (inactive) calcium dye (probe) that penetrates the cell membrane and becomes active once inside the cell. The active form of the dye becomes fluorescent after binding to intracellular calcium. Additive A is added to prevent the dye from being released out from the cell. Ligand binding stimulates GPCR activation, resulting in the release of intracellular calcium stores from the ER, which leads to an increase in fluorescence in the presence of the activated calcium dye.
Perform Rank Order Analysis of Potential Leads
Dose response and rank order potency for calcium mobilization and β-arrestin recruitment assays using the same cell line. The PathHunter CHO-K1 MRGPRX2 β-Arrestin Cell Line (Cat. No. 93-0309C2) was used to analyze both calcium mobilization with the Calcium No WashPLUS detection kit and β-arrestin recruitment.
– Readouts showing select agonists effect on (A.) calcium mobilization of intracellular calcium and on (B.) β-arrestin recruitment upon binding to MrgprX2 receptors. Both dose response curves are the mean of two replicates and show normalized data calculated from the maximum effect obtained with cortistatin-14.
– C. Table showing potency and efficacy of agonist relative to cortistatin-14. Results show good agreement with those previously published with respect to rank orders of potency agonists (Grimes et al. 2019) maintained relative to cortistatin-14 used as reference agonist for both assays.
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