Recombinant Proteins and Enzymes

Offering Industry’s Largest Portfolio of Purified Recombinant Proteins for Screening Inhibitors

Recombinant proteins in drug discovery and development are exogenous proteins expressed in a production organism and used for the treatment or prevention of disease in humans or animals. Kinases are cell signaling enzymes that catalyze the transfer of a phosphate group from ATP to its substrate, while phosphatases are regulatory enzymes that remove the phosphate group. Epigenetic proteins modify the structure of DNA or its associated proteins, such as histones, to regulate gene expression. These proteins play roles in the regulation of intracellular signaling pathways or modulate epigenetic mechanisms. Ubiquitin proteins perform key roles in protein degradation pathways and cellular processes such as DNA repair, gene expression, and endocytosis.

There are ~538 kinases in the human genome (eLife Sciences, 2021), and they are generally grouped into either serine/threonine, tyrosine, or lipid kinases. Kinase substrates include a variety of intracellular proteins and molecules, including other kinases, transcription factors, lipids, and other proteins. The phosphorylation and dephosphorylation of the substrate modifies its enzymatic activity. These activities include diverse cellular processes, including cell division, protein synthesis, transcriptional regulation, and neurotransmission. Activating mutations of kinases, or their dysregulation by upstream factors, that lead to either gain-of-function or loss-of-function are implicated in many diseases, including cancer.

Eurofins DiscoverX® provides a new home for the original Millipore (Upstate) kinase enzyme portfolio covering over 70% of the active human kinome. View the comprehensive portfolio of over 440 active, mutant, and inactive recombinant kinases available in multiple pack sizes, including bulk sizing, to provide you with scalable options. Dissect the physiological role of kinases and phosphatases, screen for inhibitors, or perform enzymatic kinases or phosphatase activity assays. Access the same proteins used daily in the renowned KinaseProfiler, PhosphataseProfiler, and EPIscan services, and receive the quality and confidence that comes with screening hundreds of millions of data points generated over the past several decades.

Product Highlights

  • Comprehensive Portfolio - Over 520 active, inactive, unactive, and mutant kinases, phosphatases, and epigenetic and ubiquitin proteins, including many exclusive enzymes along with the PI3K and the PIKK group (ATM, ATR, mTOR, and DNA-PK)
  • Optimal Expression Systems - Enzymes produced in their optimal system (insect, mammalian, or bacterial) to give the correct characteristics for activity and inhibition
  • High Quality - Rigorous quality-control procedures ensure the highest purity, specific activity, and lot-to-lot consistency
  • Multiple Sizes - Multiple pack sizes and bulk sizing available to provide you with flexible options from a one-time experiment to a full-blown HTS campaign
  • Kinases – View over 440 active, inactive, and unactivated kinases available as human, mouse, rat, and other species; expressed from bacterial, insect, or mammalian cells
  • Phosphatases – Utilize the same phosphatase, expressed in E. coli and tagged (e.g. GST, His6) or untagged, in your discovery that are used in the renowned PhosphataseProfiler drug discovery services
  • Epigenetic Proteins – Recombinant epigenetic proteins, expressed in E. coli and tagged (e.g. GST, His6), and used in EPIscan screening and profiling services (based on the novel InCELL Hunter technology)

Access Products by Target

Target Lists Products Target List

Comprehensive Eurofins DiscoverX product list to enable your drug discovery and development programs focused on checkpoint receptors, cytokine receptors/interleukins, cytotoxicity,…

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Target Lists Kinase and Phosphatase Product Solutions

Comprehensive Offering of Cellular Assays and Recombinant Enzymes for Drug Discovery Screening and Lead Optimization

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Webinars Creating Cellular Target Engagement Assays for Diverse Kinases Using InCELL Pulse

Kinase inhibitors often potently inhibit several off-target kinases in biochemical assays. Unfortunately, the relevance of these off-target interactions in a…

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Videos Custom Development Capabilities

Learn about Eurofins DiscoverX cell line engineering, assay development, and recombinant enzyme production custom capabilities. Find out more about cell…

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Brochures & Flyers Expedite Cancer Drug Discovery & Development

Functional Cell-Based Assays, Cell Lines, & Proteins for Cancer Research Improved or novel small molecule or biologic therapeutics for cancer…

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Brochures & Flyers High-Throughput Screening & Lead Optimization Products

Robust Cell-Based Assays to Identify Therapeutic Hits Rapidly

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Brochures & Flyers Eurofins DiscoverX – Delivering Solutions from Discovery to Market

Products & Custom Development Capabilities.

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Kinase Product Solutions

Enzymes & cell-based functional, binding, & activity assays for discovery through development & for QC lot-release testing

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Epigenetic Proteins Product Solutions

Recombinant proteins & cell-based assays to identify small molecule inhibitors & modifiers of methyltransferases & bromodomains

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ADP Accumulation Assays

Antibody-free, homogeneous assays to monitor ATP depletion & ADP accumulation in cells for kinase profiling applications

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Receptor Tyrosine Kinase Assays

Functional & activity assays to analyze receptor dimerization, phosphorylation, internalization, & SH2-recruitment of RTKs & CTK/cytokine receptors

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Target Engagement Assays

Cell-based assays to confirm compound cell entry, protein target binding, screen & rank inhibitors, & measure cellular potencies

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Custom Capabilities

Custom cell lines, kits, assays, & protein development capabilities optimized to fit your requirements

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