Cell Lines and Primary Cells

Engineered cell lines and primary cells are important tools used for in vitro cell-based assays that replicate the physiology of biological systems. Primary cells are isolated or derived directly from living tissues, and retain morphological and functional characteristics of their source. Cell lines are derived or engineered from primary cells and can be propagated repeatedly and kept in culture for longer time periods. These cells provide the cost-effective ability to deliver more physiologically relevant results reproducibly.

Eurofins DiscoverX® provides the largest collection of fully qualified, functional whole cell assays that enable unique drug discovery and development solutions at every stage of your research, with over two decades of experience in generating stable cell lines of unsurpassed quality and industry-validated performance. These cell lines have been used to screen billions of data points including screening daily by Eurofins Discovery, and they have been used to bring many therapeutics to the market.

Eurofins DiscoverX offers over 1,300 stable cell lines, cell pools, frozen cells, and primary immune effector cells for a broad range of mechanism of action (MOA) reflective target-based assays for target classes including GPCRs, kinases, cytokines, checkpoint inhibitors, ion channels, epigenetics, nuclear proteins, and several applications such as for cytotoxicity studies.

Use the application-specific detection kits with your appropriate cell line model for a complete ready-to-use, functional cell-based assay to interrogate the MOA of your small molecule or biologic, measure and rank ligands potencies, perform binding and functional screens, implement as lot release assay, and much more.

Product Highlights

  • Robust Performance – Rigorously and continuously validated for accurate pharmacology through Eurofins Discovery with billions of data points screened
 
  • Versatile Use – Interrogate diverse MOAs with broad applications ideal for small molecules and biologics screening or profiling
 
  • Diverse – Select relevant cell models from over 50 cell types such as HEK 293, CHO-K1, U2OS, and more
 
  • Established – Implemented in thousands of screening programs and over 50 lot release programs for biologics
  • Stable Cell Lines – Stable cell lines extensively validated with billions of compounds, confirmed stability beyond 10 passages, and usable for a variety of research and drug discovery and development applications
  • KILR® Effector Cells – Single donor-derived human primary cytotoxic T cells to measure target cell death using antibody-dependent cell-mediated cytotoxicity and bi-specific antibody-mediated T Cell Redirection assays for screening, characterization, and QC lot release of antibody drugs
  • KILR Cell Lines and Pools – Stable cell lines and cell pools to specifically measure target cell death in co-cultures using a simple, non-radioactive, and dye-free method that can be easily implemented in drug discovery through QC lot release programs
  • GPCR cAMP and Calcium Cells – Ready-to-assay GPCR cryo-preserved cells validated for cAMP accumulation and calcium flux applications and ideal for screening of agonists, antagonists and various modulators
  • Parental Cell Lines – Engineered cells to generate your own assays to measure internalization, β-arrestin recruitment, pharmacotrafficking, and translocation assays of your membrane protein of interest, as well as receptor-specific signaling pathway activation

Eurofins DiscoverX has over 2 decades of experience engineering and developing cell lines with diverse cell backgrounds, expression systems, and reporters.

  • A wide selection of diverse cell backgrounds (e.g. HEK 293, CHO, U2OS, 1321N1, Jurkat, Raji, Daudi, and more) in their appropriate adherent or suspension cell models
  • Engineered to accurately represent the target biology
  • Extensively evaluated target expression using by qPCR, flow cytometry, immunofluorescence, or Western blotting
  • Receptor expression and performance stability confirmed for a minimum of 20 passages
  • Implement as single cell or co-culture models as relevant to the drug’s MOA
  • Vector development using a viral delivery systems based on retrovirus or lentivirus
  • Engineered systems using heterologous expression systems with either constitutive (e.g. CMV) or inducible (e.g. tetracycline) promoters
  • CRISPR/Cas9 capabilities for knockouts and knock-ins
  • Multiple reporter systems for functional readouts including Enzyme Fragment Complementation (EFC) with luminescent read-out

Target Lists Products Target List

Comprehensive Eurofins DiscoverX product list to enable your drug discovery and development programs focused on checkpoint receptors, cytokine receptors/interleukins, cytotoxicity,…

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Target Lists GPCR Product Solutions

Your source for a complete offering of GPCR assays, cell lines, and membrane preps

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Target Lists Ion Channel Product Solutions

Functionally validated ion channel products for drug discovery and safety pharmacology

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Target Lists Kinase and Phosphatase Product Solutions

Comprehensive Offering of Cellular Assays and Recombinant Enzymes for Drug Discovery Screening and Lead Optimization

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Target Lists Cell-based Assays for Biologics Development Programs

Accelerate your biologics development from discovery to QC lot release with cell-based assays. Comprehensive list of bioassay kits, cell lines,…

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Videos Custom Development Capabilities

Learn about Eurofins DiscoverX cell line engineering, assay development, and recombinant enzyme production custom capabilities. Find out more about cell…

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Brochures & Flyers High-Throughput Screening & Lead Optimization Products

Robust Cell-Based Assays to Identify Therapeutic Hits Rapidly

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Brochures & Flyers Eurofins DiscoverX – Delivering Solutions from Discovery to Market

Products & Custom Development Capabilities.

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GPCR Product Solutions

Cell-based assays to measure 2nd messengers (cAMP & calcium), β-arrestin recruitment, receptor internalization, & ligand binding

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Kinase Product Solutions

Enzymes & cell-based functional, binding, & activity assays for discovery through development & for QC lot-release testing

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Cytokine Receptor Product Solutions

Comprehensive portfolio of cell-based, functional assays for cytokines & interleukin receptors

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Checkpoint Receptor Assays

Accelerate cancer immunotherapy drug development with easy-to-use, functional cell-based assays

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Target Engagement Assays

Cell-based assays to confirm compound cell entry, protein target binding, screen & rank inhibitors, & measure cellular potencies

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Signaling Pathway Product Solutions

Interrogate a variety of popular target-specific pathways with cell-based assays

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KILR® Cytotoxicity Product Solutions

Simple, non-radioactive, & dye-free cytotoxicity assays to specifically measure target cell death

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Build Your Own Assay Tools

Complete set of parental cell lines, vectors, kits, & retroparticles to build your own stable cell lines & cell-based assays

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Epigenetic Proteins Product Solutions

Recombinant proteins & cell-based assays to identify small molecule inhibitors & modifiers of methyltransferases & bromodomains

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Nuclear Protein Product Solutions

Cell-based assays to evaluate activation & translocation of nuclear proteins including nuclear hormone receptors

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Ion Channel Solutions

High-quality ion channel cell lines for target discovery, hit screening, lead optimization, & safety studies

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Custom Capabilities

Custom cell lines, kits, assays, & protein development capabilities optimized to fit your requirements

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