PathHunter® eXpress TACR2 Activated GPCR Internalization Assay

Awaiting product image

The PathHunter® eXpress TACR2 Activated GPCR Internalization Assay measures TACR2 (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0677E3CP0L

$1,789.00 – $5,989.00

Inquire
Biological Information
Background Information:
The PathHunter® TACR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. tachykinin, substance P) based internalization of TACR2 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged TACR2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting TACR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Tachykinin Receptors
Sub Family:
Class A
Coupling:
Gq
Accession Number:
AY322545
Target Name:
TACR2
Target Aliases:
Tachykinin receptor 2, NK2R, NKNAR, SKR, TAC2R
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Internalization
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-261 PathHunter eXpress Activated GPCR Internalization Assays REV7 User Manual

View Document

Datasheets

93-0677E3x Datasheet

View Document