PathHunter® eXpress mCXCR4 U2OS β-Arrestin GPCR Assay

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The PathHunter® eXpress mCXCR4 U2OS β-Arrestin GPCR Assay measures mCXCR4 (GPCR) activity via recruitment of β-Arrestin 2. eXpress kits contain all assay materials: cells, reagents, and plates. Mouse version.

Catalog #: 93-0481E3MCP5L

$1,789.00 – $5,989.00

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Biological Information
Background Information:
The PathHunter® mCXCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of mCXCR4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged mCXCR4 and EA-tagged β-Arrestin-2. Activation of mCXCR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein.
Target Class:
GPCR
Family:
Chemokine Receptors
Coupling:
Gi/Go
Accession Number:
NM_009911.2
Target Name:
CXCR4 (SDF-1R)
Target Aliases:
Chemokine (C-X-C motif) receptor 4, CD184, CXC-R4, CXCR-4, Cmkar4, LESTR, PB-CKR, PBSF/SDF-1, Sdf1r, b2b220Clo
Target Species:
Mouse
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-255 PathHunter B Arrestin eXpress GPCR Assay REV4 User Manual

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Datasheets

93-0481E3x Datasheet

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