| Biological Information | |
|---|---|
| Background Information: | The PathHunter® IL18R1/IL18RAP Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-18) induced dimerization of IL18R1/IL18RAP receptors. This product uses EFC technology. The cells included overexpress IL18R1 target protein fused to PK alongside IL18RAP target protein fused to EA. Upon ligand-induced dimerization of IL18R1/IL18RAP, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | Cytokine Receptor |
| Family: | Immunoglobulin-like family of IL-1 Receptors |
| Accession Number: | NM_003855.3 |
| Target Name: | IL18R1/IL18RAP |
| Target Aliases: | interleukin 18 receptor, CD218a, CDw218a, IL-18R-alpha, IL-18Ralpha, IL-1Rrp, IL18RA, IL18Ralpha2, IL1RRP, ACPL, CD218b, CDw218b, IL-18R-beta, IL-18RAcP, IL-18Rbeta, IL-1R-7, IL-1R7, IL-1RAcPL, IL18RB |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Dimerization |
| Detection Method: | Chemiluminescence |
| Clinical Relevance | |
| Therapeutic Area: | Inflammation/Allergy |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® eXpress IL18R1/IL18RAP Dimerization Assay
The PathHunter® eXpress IL18R1/IL18RAP Dimerization Assay measures ligand-induced dimerization of IL18R1/IL18RAP. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-301 PathHunter eXpress Dimerization Assay Kit REV3 User Manual
View DocumentDatasheets
93-1044E3x Datasheet
View Document