Biological Information | |
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Background Information: | The PathHunter® HTR1B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1B GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged HTR1B and EA-tagged β-Arrestin-2. Activation of HTR1B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | 5-Hydroxytryptamine Receptors (Serotonin) |
Sub Family: | Class A |
Coupling: | Gi/Go |
Accession Number: | NM_000863 |
Target Name: | HTR1B (5-HT1B receptor) |
Target Aliases: | 5-hydroxytryptamine receptor 1B, 5-HT-1B, 5-HT-1D-beta, 5-HT1B, 5-HT1DB, 5-hydroxytryptamine (serotonin) receptor 1B, 5-hydroxytryptamine (serotonin) receptor 1B, G protein-coupled, 5-hydroxytryptamine receptor 1B, HTR1D2, HTR1DB, S12, serotonin 1D beta receptor, serotonin receptor 1B |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
β-Arrestin isoform: | β-Arrestin-2 |
Assay Measures: | β-Arrestin Recruitment |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® eXpress HTR1B U2OS β-Arrestin GPCR Assay
The PathHunter® eXpress HTR1B U2OS β-Arrestin GPCR Assay measures HTR1B (GPCR) activity via recruitment of β-Arrestin 2. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-255 PathHunter B Arrestin eXpress GPCR Assay REV4 User Manual
View DocumentDatasheets
93-0697E3x Datasheet
View Document