Biological Information | |
---|---|
Background Information: | The PathHunter® Flt3 Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activation via detection of resulting Flt3 phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress a PK-tagged engineered fusion of the catalytic domain of Flt3 to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Target Class: | Kinase |
Family: | Receptor Tyrosine Kinase |
Sub Family: | Protein Tyrosine |
Accession Number: | NM_005228.3 |
Target Name: | FLT3 |
Target Aliases: | FLT3, fms related receptor tyrosine kinase 3, FLK2, STK1, CD135, FLK-2 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Functional Activity |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® eXpress Flt3 Activity Assay
The PathHunter® eXpress Flt3 Activity assay measures Flt3 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-299 PathHunter eXpress Receptor Tyrosine Kinase Activity Assay Kit REV3 User Manual
View DocumentDatasheets
93-0901E3x Datasheet
View Document