| Biological Information | |
|---|---|
| Background Information: | The PathHunter® eXpress FGFR4-β-Klotho Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated FGFR4 and β-Klotho dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, β-Klotho Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | Kinase |
| Family: | Receptor Typosine Kinase (RTK): Growth Factor Receptor |
| Sub Family: | Protein Tyrosine |
| Accession Number: | NM_002011 |
| Target Name: | FGFR4-β-Klotho |
| Target Aliases: | Fibroblast growth factor receptor 4, TKF, JTK2, CD334 |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Functional Activity |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® eXpress FGFR4-β-Klotho Functional Assay
The PathHunter® eXpress FGFR4-β-Klotho Functional Assay measures FGFR4-β-Klotho receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-270 PathHunter eXpress Receptor Tyrosine Kinase Functional Assay Kit REV9 User Manual
View DocumentDatasheets
93-1060E3x Datasheet
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