Biological Information | |
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Background Information: | The PathHunter® CXCR7 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. CXCL12/SDF-1α) based internalization of CXCR7 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CXCR7. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CXCR7-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | Chemokine Receptors |
Sub Family: | Class A |
Accession Number: | NM_020311.2 |
Target Name: | CXCR7 (CMKOR1) |
Target Aliases: | Chemokine (C-X-C motif) receptor 7, CMKOR1, CXC-R7, CXCR-7, CXCR7, GPR159, RDC-1, RDC1 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Internalization |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® eXpress CXCR7 Activated GPCR Internalization Assay
User Manuals & Protocols
70-261 PathHunter eXpress Activated GPCR Internalization Assays REV7 User Manual
View DocumentDatasheets
93-0738E3_CXCR7_011224
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