Biological Information | |
---|---|
Background Information: | The PathHunter® CXCR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. CXCL8/IL-8) based internalization of CXCR2 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CXCR2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CXCR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | Chemokine Receptors |
Sub Family: | Class A |
Coupling: | Gi/Go |
Accession Number: | NM_001557 |
Target Name: | CXCR2 (IL8RB) |
Target Aliases: | Chemokine (C-X-C motif) receptor 2, CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB, WHIMS2 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Internalization |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® eXpress CXCR2 Activated GPCR Internalization Assay
The PathHunter® eXpress CXCR2 Activated GPCR Internalization Assay measures CXCR2 (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-261 PathHunter eXpress Activated GPCR Internalization Assays REV7 User Manual
View DocumentDatasheets
93-0934E3x Datasheet
View Document