| Biological Information | |
|---|---|
| Background Information: | The PathHunter® CHRM3 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. acetylcholine) based internalization of CHRM3 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged CHRM3 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CHRM3 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | Acetylcholine Receptor (Muscarinic) |
| Sub Family: | Class A |
| Coupling: | Gq |
| Accession Number: | AF498917 |
| Target Name: | CHRM3 |
| Target Aliases: | Cholinergic receptor, muscarinic 3, CHRM3, M3, Acetylcholine (Muscarinic) |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Internalization |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® eXpress CHRM3 Total GPCR Internalization Assay
The PathHunter® eXpress CHRM3 Total GPCR Internalization Assay measures CHRM3 (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-262 PathHunter eXpress Total GPCR Internalization Assays REV7 (1) User Manual
View DocumentDatasheets
93-0775E3x Datasheet
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