| Biological Information | |
|---|---|
| Background Information: | The PathHunter® CCR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. CCL2/MCP-1) based internalization of CCR2 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged CCR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | Chemokine Receptors |
| Sub Family: | Class A |
| Coupling: | Gi/Go |
| Accession Number: | NM_001123396 |
| Target Name: | CCR2 (MIP-1R) |
| Target Aliases: | Chemokine (C-C motif) receptor 2, Cc-ckr-2a, Ccr2b, Ckr2, Ckr2a, Ckr2b, Cmkbr2, mJe-r, Ccr2 |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Internalization |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® eXpress CCR2 Total GPCR Internalization Assay
The PathHunter® eXpress CCR2 Total GPCR Internalization Assay measures CCR2 (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-262 PathHunter eXpress Total GPCR Internalization Assays REV7 (1) User Manual
View DocumentDatasheets
93-0679E3x Datasheet
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