PathHunter® eXpress AGTRL1 Activated GPCR Internalization Assay

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The PathHunter® eXpress AGTRL1 Activated GPCR Internalization Assay measures AGTRL1 (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0710E3CP0L

$1,789.00 – $5,989.00

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Biological Information
Background Information:
The PathHunter® AGTRL1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. apelin-13) based internalization of AGTRL1 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged AGTRL1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting AGTRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Apelin Receptor
Sub Family:
Class A
Coupling:
Gi/Go
Accession Number:
NM_005161
Target Name:
AGTRL1
Target Aliases:
APLNR, Apelin receptor, APJ, APJR, HG11, AGTRL1
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Internalization
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-261 PathHunter eXpress Activated GPCR Internalization Assays REV7 User Manual

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Datasheets

93-0710E3x Datasheet

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