| Biological Information | |
|---|---|
| Background Information: | The PathHunter® Epoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoietin or Epoetin Alfa-induced dimerization of EPOR receptors. This product uses EFC technology. The included cells overexpress EPOR target protein fused to PK alongside EPOR target protein fused to EA. Upon ligand-induced dimerization of EPOR, detectable EFC signal is produced. This assay has been optimized and qualified with Aranesp® (not supplied in the bioassay kit). Aranesp® is a registered trademark of Amgen Inc. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | Kinase |
| Family: | Prolactin Receptor Family |
| Accession Number: | NM_000121.3 |
| Target Name: | EPOR |
| Target Aliases: | EPO-R, Erythropoietin receptor |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Dimerization |
| Detection Method: | Chemiluminescence |
| Bioassay Data | |
| Qualified With: | Aranesp® |
| Trademark Statement: | Aranesp® is a registered trademark of Amgen Inc. |
| Clinical Relevance | |
| Therapeutic Area: | Oncology/Immuno-Oncology |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® Epoetin Alfa Bioassay Kit
The PathHunter® Epoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoietin or Darbepoetin Alfa-induced dimerization of EPOR receptors.
View bioassay qualification data.
User Manuals & Protocols
70-315 PathHunter Epoetin Alfa Bioassay Kit REV3 User Manual
View DocumentDatasheets
93-0965Y3x Datasheet
View Document