| Biological Information | |
|---|---|
| Background Information: | The InCELL Hunter™ eXpress CSNK1D Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of CSNK1D via detection of CSNK1D levels. This product uses EFC technology. The cells included overexpress CSNK1D target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | Kinase |
| Family: | casein kinase 1 (CK1) family |
| Sub Family: | Protein Ser/Thr |
| Accession Number: | NC_000017.11 |
| Target Name: | CSNK1D |
| Target Aliases: | ASPS, CKId, HCKID, FASPS2, CKIdelta, CKI-delta |
| Target Species: | Human |
| Cell Background: | A549 |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Target Engagement |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Target Engagement |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | InCELL Hunter™ |
InCELL Hunter™ eXpress CSNK1D Kinase Binding Assay
The InCELL Hunter™ eXpress CSNK1D Kinase Binding Assay measures target engagement via compound-induced stabilization of CSNK1D target protein. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-429 InCELL Hunter eXpress Assays User Manual REV0A
View DocumentDatasheets
96-0048E15x Datasheet
View Document