ChemiBrite™ EP3 Prostanoid Receptor Stable Cell Line

Awaiting product image

The ChemiBRITE™ EP3 Prostanoid Receptor Stable Cell Line measures PTGER3 (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.

Catalog #: HTS092L Size: 2 vials
Inquire
Biological Information
Background Information:
The ChemiBrite EP3 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER3 , the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Prostanoid Receptors
Accession Number:
NM_198716
Target Name:
PTGER3
Target Aliases:
Prostaglandin E receptor 3 (subtype EP3), EP3, EP3-I, EP3-II, EP3-III, EP3-IV, EP3-VI, EP3e, PGE2-R, lnc003875, PTGER3, EP3, Prostanoid
Target Species:
Human
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Additional Information
Brand:
ChemiBrite™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

View Document

Datasheets

HTS092L Datasheet

View Document