Biological Information | |
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Background Information: | The cAMP Hunter™ RXFP3 (SALPR) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of RXFP3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type RXFP3, a naturally Gi/Go coupled GPCR. Upon activation of RXFP3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | Relaxin Family Peptide Receptors |
Sub Family: | Class A |
Coupling: | Gi/Go |
Accession Number: | NM_016568.2 |
Target Name: | RXFP3 (SALPR) |
Target Aliases: | Relaxin family peptide receptor 3, G-protein coupled receptor GPCR135, G-protein coupled receptor SALPR, GPCR135, RLN3 receptor 1, RLN3R1, RXFPR3, SALPR, g protein-coupled receptor SALPR, relaxin 3 receptor 1, relaxin family peptide receptor 3, relaxin-3 receptor 1, relaxin/insulin-like family peptide receptor 3, somatostatin and angiotensin-like peptide receptor, somatostatin- and angiotensin-like peptide receptor |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | cAMP Detection |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | cAMP Hunter™ |
cAMP Hunter™ eXpress RXFP3 (SALPR) CHO-K1 GPCR Assay
The cAMP Hunter™ eXpress RXFP3 (SALPR) CHO-K1 GPCR Assay measures RXFP3 (GPCR) activity via decreased levels of intracellular cAMP. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-234 cAMP Hunter eXpress GPCR Assay REV10 User Manual
View DocumentDatasheets
95-0102E2x Datasheet
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