| Biological Information | |
|---|---|
| Background Information: | The cAMP Hunter™ CXCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of CXCR4 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR4, a naturally Gi/Go coupled GPCR. Upon activation of CXCR4, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | Chemokine Receptors |
| Sub Family: | Class A |
| Coupling: | Gi/Go |
| Accession Number: | AY242129 |
| Target Name: | CXCR4 (SDF-1R) |
| Target Aliases: | Chemokine (C-X-C motif) receptor 4, CXCR4, CXCR4, Chemokine |
| Target Species: | Human |
| Cell Background: | CHO-K1 |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | cAMP Detection |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | cAMP Hunter™ |
cAMP Hunter™ eXpress CXCR4 CHO-K1 GPCR Assay
The cAMP Hunter™ eXpress CXCR4 CHO-K1 GPCR Assay measures CXCR4 (GPCR) activity via decreased levels of intracellular cAMP. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-234 cAMP Hunter eXpress GPCR Assay REV10 User Manual
View DocumentDatasheets
95-0081E2x Datasheet
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