| Biological Information | |
|---|---|
| Background Information: | The cAMP Hunter™ ADRA2A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRA2A, a naturally Gi/Go coupled GPCR. Upon activation of ADRA2A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | Adrenoceptor |
| Sub Family: | Class A |
| Coupling: | Gi/Go |
| Accession Number: | NM_000681 |
| Target Name: | ADRA2A (α2A-adrenoceptor) |
| Target Aliases: | ADRA2, ADRA2R, ADRAR, ALPHA2AAR, ZNF32, Adrenergic receptor alpha 2A, ADRA2A, α2A, Adrenoceptor |
| Target Species: | Human |
| Cell Background: | CHO-K1 |
| Usage | |
| Product Type: | eXpress Assay Kits |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | cAMP Detection |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | cAMP Hunter™ |
cAMP Hunter™ eXpress ADRA2A CHO-K1 GPCR Assay
The cAMP Hunter™ eXpress ADRA2A CHO-K1 GPCR Assay measures ADRA2A (GPCR) activity via decreased levels of intracellular cAMP. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-234 cAMP Hunter eXpress GPCR Assay REV10 User Manual
View DocumentDatasheets
95-0126E2_cAMP_Hunter_eXpress_ADRA2A_ CHO-K1_GPCR_Assay_DS.02212024
View Document