HitHunter® Calcium No WashPLUS Assay Platform

DiscoveRx’s HitHunter Calcium No Wash^PLUS kits use a homogenous assay format to detect calcium mobilization. Measurement of intracellular calcium provides valuable information on the activation status of GPCRs and ion channels. Stimulated receptor releases intracellular calcium, causing the calcium-sensitive dye in the kit to fluoresce. Our calcium assay kits provide a fast, reliable, low-cost alternative to traditional wash and quench-based assays.

HitHunter® Calcium Assay Principle

Aequorin-based Calcium Assays

All aequorin-based luminescence assays require addition of a coelenterazine substrate. DiscoveRx offers an optimized form called Coelenterazine H (90-0084).  This derivative of native coelenterazine is significantly more sensitive to calcium and provides enhanced performance in aequorin-based luminescent HTS calcium assays.

• VIEW CALCIUM ASSAYS
• Biochemical Assays
• Ligands, Inhibitors & Agonists

Calcium Flux Analysis Using HitHunter® Calcium No Wash^PLUS Assay

Adrenergic alpha 1B receptor		EDG3
Uncover unique pharmacologies for all classes of compounds. Depicted are dose curves identifying responses to various agonists for the Adrenergic alpha 1B receptor [click graph to enlarge].		EDG3 cells (squares) and native parental cells (triangles) were stimulated with a control agonist and calcium influx was detected with FLIPR® Tetra [click graph to enlarge]

Features & Benefits

• Single step, no wash protocol
• Large assay windows, larger dynamic range and  improved signal & performance over FLUO-3
• Flexible protocol - use with adherent or non-adherent cells, GPCRs & calcium channels
• Fewer false positives, due to reduced spontaneous calcium flux and minimal perturbation of cells
• Validated for use with all Gq-coupled PathHunter® cell lines