Biological Information | |
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Background Information: | The GPR39 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. Zn2+) based activation of GPR39 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GPR39 , a naturally Gq coupled GPCR. Upon activation of GPR39 , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Class A Orphan |
Sub Family: | Class A |
Coupling: | Gq |
Accession Number: | NM_001508 |
Target Name: | GPR39 |
Target Aliases: | G protein-coupled receptor 39, G-protein coupled receptor 39 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Calcium Flux |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | DiscoverX |
U2OS GPR39 Gq Cell Line
The U2OS GPR39 Gq Cell Line measures GPR39 (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-296 Native Calcium GPCR Assays REV2 User Manual
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95-1015C3 Datasheet
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