| Biological Information | |
|---|---|
| Background Information: | The Ready-to-Assay™ S1P3 Lysophospholipid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. S1P) based activation of S1PR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress S1PR3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of S1PR3 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. |
| Target Class: | GPCR |
| Family: | Lysophospholipid (LPA) Receptors |
| Accession Number: | NM_005226 |
| Target Name: | S1PR3 |
| Target Aliases: | S1PR3, S1P3/EDG3, Lysophospholipid (LPA & S1P) |
| Target Species: | Human |
| Usage | |
| Product Type: | Frozen Cells |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Measures: | Calcium Flux |
| Detection Method: | Fluorescence |
| Additional Information | |
| Brand: | ChemiSCREEN™ |
Ready-to-Assay™ S1P3 Lysophospholipid Receptor Frozen Cells
The Ready-to-Assay™ S1P3 Lysophospholipid Receptor Frozen Cells measure S1PR3 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
View DocumentDatasheets
HTS097RTA Datasheet
View Document