Ready-to-Assay™ GPBA Bile Acid Receptor Frozen Cells

The Ready-to-Assay™ GPBA Bile Acid Receptor Frozen Cells measure GPBAR1 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.

Catalog #: HTS238LRTA Size: 2 vials

Biological Information
Background Information:	The Ready-to-Assay™ GPBA Bile Acid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. [ligand]) based activation of GPBAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress GPBAR1 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of GPBAR1 , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.
Target Class:	GPCR
Family:	Bile Acid Receptor (GPBA)
Target Name:	GPBAR1
Target Aliases:	GPBAR1, GPR131, Bile Acid (GPBA)
Target Species:	Human

Usage
Product Type:	Frozen Cells
Application:	Drug Discovery & Development
Storage Conditions:	Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Measures:	Calcium Flux
Detection Method:	Fluorescence

Additional Information
Brand:	ChemiSCREEN™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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Datasheets

HTS238LRTA Datasheet

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