Biological Information | |
---|---|
Background Information: | The Ready-to-Assay™ FPRL1 N-Rormylpeptide Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. N-formyl peptides) based activation of FPR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress FPR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of FPR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. |
Target Class: | GPCR |
Family: | Formylpeptide Receptors |
Accession Number: | NM_001462 |
Target Name: | FPR2 |
Target Aliases: | FPR2, FPRL1/ALX, Formylpeptide |
Target Species: | Human |
Usage | |
Product Type: | Frozen Cells |
Application: | Drug Discovery & Development |
Storage Conditions: | Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Measures: | Calcium Flux |
Detection Method: | Fluorescence |
Additional Information | |
Brand: | ChemiSCREEN™ |
Ready-to-Assay™ FPRL1 N-Rormylpeptide Receptor Frozen Cells
The Ready-to-Assay™ FPRL1 N-Rormylpeptide Receptor Frozen Cells measure FPR2 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
View DocumentDatasheets
HTS056RTA Datasheet
View Document