| Biological Information | |
|---|---|
| Background Information: | The Ready-to-Assay™ FFA1/GPR40 Free Fatty Acid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. free fatty acids) based activation of FFAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress FFAR1 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FFAR1 , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. |
| Target Class: | GPCR |
| Family: | Free fatty Acid Receptors |
| Accession Number: | NM_005303 |
| Target Name: | FFAR1 (GPR40) |
| Target Aliases: | free fatty acid receptor 1, FFAR1, FFA1R, GPR40, GPCR40, FFA1/GPR40, Free Fatty Acid |
| Target Species: | Human |
| Usage | |
| Product Type: | Frozen Cells |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Measures: | Calcium Flux |
| Detection Method: | Fluorescence |
| Additional Information | |
| Brand: | ChemiSCREEN™ |
Ready-to-Assay™ FFA1/GPR40 Free Fatty Acid Receptor Frozen Cells
The Ready-to-Assay™ FFA1/GPR40 Free Fatty Acid Receptor Frozen Cells measure FFAR1 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
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HTS038LRTA Datasheet
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