Biological Information | |
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Background Information: | The Ready-to-Assay™ CXCR4 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL12/SDF-1α) based activation of CXCR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CXCR4 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR4 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. |
Target Class: | GPCR |
Family: | Chemokine Receptors |
Accession Number: | M99293 |
Target Name: | CXCR4 (SDF-1R) |
Target Aliases: | Chemokine (C-X-C motif) receptor 4, CXCR4, CXCR4, Chemokine |
Target Species: | Human |
Cell Background: | Chem-1 |
Usage | |
Product Type: | Frozen Cells |
Application: | Drug Discovery & Development |
Storage Conditions: | Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Measures: | Calcium Flux |
Detection Method: | Fluorescence |
Additional Information | |
Brand: | ChemiSCREEN™ |
Ready-to-Assay™ CXCR4 Chemokine Receptor Frozen Cells
The Ready-to-Assay™ CXCR4 Chemokine Receptor Frozen Cells measure CXCR4 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
View DocumentDatasheets
HTS004RTA Datasheet
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