Ready-to-Assay™ CXCR3 Chemokine Receptor Frozen Cells

The Ready-to-Assay™ CXCR3 Chemokine Receptor Frozen Cells measure CXCR3 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.

Catalog #: HTS003RTA Size: 2 vials

Biological Information
Background Information:	The Ready-to-Assay™ CXCR3 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CXCR3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR3 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.
Target Class:	GPCR
Family:	Chemokine Receptors
Accession Number:	NM_001504.1
Target Name:	CXCR3 (IP-10)
Target Aliases:	Chemokine (C-X-C motif) receptor 3, CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR
Target Species:	Human
Cell Background:	Chem-1

Usage
Product Type:	Frozen Cells
Application:	Drug Discovery & Development
Storage Conditions:	Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Measures:	Calcium Flux
Detection Method:	Fluorescence

Additional Information
Brand:	ChemiSCREEN™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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Datasheets

HTS003RTA Datasheet

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