Ready-to-Assay™ CCR10 Chemokine Receptor Frozen Cells

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The Ready-to-Assay™ CCR10 Chemokine Receptor Frozen Cells measure CCR10 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.

Catalog #: HTS014RTA Size: 2 vials

$849.00

Biological Information
Background Information:
The Ready-to-Assay™ CCR10 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CCL2, MCP-1) based activation of CCR10 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CCR10 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR10 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.
Target Class:
GPCR
Family:
Chemokine Receptors
Accession Number:
AF215981
Target Name:
CCR10
Target Aliases:
Chemokine (C-C motif) receptor 10, CCR10, CCR10, Chemokine
Target Species:
Human
Cell Background:
Chem-1
Usage
Product Type:
Frozen Cells
Application:
Drug Discovery & Development
Storage Conditions:
Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Additional Information
Brand:
ChemiSCREEN™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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Datasheets

HTS014RTA Datasheet

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