Ready-to-Assay™ alpha1D Adrenergic Receptor Frozen Cells

The Ready-to-Assay™ α1D Adrenergic Receptor Frozen Cells measure ADRA1D (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.

Catalog #: HTS216RTA Size: 2 vials

Biological Information
Background Information:	The Ready-to-Assay™ α1D Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1D GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress ADRA1D (lacking residues 2-79) alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA1D , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.
Target Class:	GPCR
Family:	Adrenoceptor
Accession Number:	NM_000678
Target Name:	ADRA1D (α1D-adrenoceptor)
Target Aliases:	Adrenergic receptor alpha 1D, DAR, ADRA1, ADRA1A, ADRA1R, ALPHA1
Target Species:	Human

Usage
Product Type:	Frozen Cells
Application:	Drug Discovery & Development
Storage Conditions:	Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Measures:	Calcium Flux
Detection Method:	Fluorescence

Additional Information
Brand:	ChemiSCREEN™

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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Datasheets

HTS216RTA Datasheet

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