RBL-2H3 MRGPRX1 Gq Cell Line

The RBL-2H3 MRGPRX1 Gq Cell Line measures MRGPRX1 (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.

Catalog #: 95-1019C11 Size: 2 vials

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Biological Information
Background Information:	The MRGPRX1 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. BAM (8-22)) based activation of MRGPRX1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MRGPRX1 , a naturally Gq coupled GPCR. Upon activation of MRGPRX1 , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Class A Orphans
Sub Family:	Class A
Coupling:	Gq
Accession Number:	NM_147199
Target Name:	MRGPRX1
Target Aliases:	MAS-related GPR, member X1
Target Species:	Human
Cell Background:	RBL-2H3

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Calcium Flux
Detection Method:	Chemiluminescence

Additional Information
Brand:	DiscoverX