| Biological Information | |
|---|---|
| Background Information: | DiscoverX cell-based assays feature the powerful in vivo application of the established Enzyme Fragment Complementation technology. In this approach, two complementing fragments (ProLink, a small enzyme donor peptide fragment, and EA, a larger enzyme acceptor fragment) of the β-galactosidase (β-gal) enzyme are expressed as protein fusions in stably transfected, clonally derived cells. The pCMV-ProLink™ cloning vectors are intended for cloning a GPCR or other protein of interest with ProLink as a C-terminal tag, and subsequently transfecting into a parental cell line containing an EA-tagged protein. ProLink 1 peptide tag has low affinity for EA and is useful for strong protein:protein interactions, while ProLink 2 peptide tag has a higher affinity (~3-fold greater) for EA and is useful for weak protein:protein interactions. ARMS (Arrestin Recruitment Modifying Sequence) ProLink tags contain GRK (G-protein Receptor Kinase) consensus phosphorylation sites and can improve assays that have a low signal-to-background ratio. |
| Target Name: | ARMS2 |
| Usage | |
| Product Type: | Vectors |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store at –20°C until use. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | ProLink™ |
pCMV-ARMS2-ProLink™ 2 Vector
The pCMV-ProLink cloning vectors are for cloning a GPCR or other protein of interest with ProLink as a C-terminal tag, and subsequently transfecting into a parental cell line containing an EA-tagged protein. The ProLink 2 peptide tag has a higher affinity (~3-fold greater) for EA. ARMS ProLink tags contain GRK consensus phosphorylation sites and can improve assays with low S:B ratios.
User Manuals & Protocols
70-385 ProLabel and ProLink Mammalian Cloning Vectors REV1 User Manual
View DocumentDatasheets
93-0490 Datasheet
View Document