Biological Information | |
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Background Information: | The PathHunter® U2OS TrkA-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkA and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged TrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, P75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Kinase |
Family: | Receptor Tyrosine Kinase |
Accession Number: | NM_002529 |
Target Name: | TrkA-P75 |
Target Aliases: | Neurotrophic tyrosine kinase receptor type 1, MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Functional Activity |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS TrkA-P75 Functional Assay
The PathHunter® U2OS TrkA-P75 Functional Assay measures TrkA-P75 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-269 PathHunter Receptor Tyrosine Kinase Functional Assay REV4 User Manual
View DocumentDatasheets
DRx_DS_93-0529C3_PHCb-RTK_U2OS_TrkA-P75_20231013
View Document