PathHunter® U2OS rTrkA-rP75 Functional Assay

The PathHunter® U2OS rTrkA-rP75 Functional Assay measures rTrkA-rP75 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells. Rat version.

Catalog #: 93-1014C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® U2OS rTrkA-rP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rTrkA and rP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged rTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, rP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rat version of the protein.
Target Class:	Kinase
Family:	Receptor Tyrosine Kinase
Accession Number:	NM_021589
Target Name:	rTrkA-rP75
Target Aliases:	Neurotrophic tyrosine kinase receptor type 1
Target Species:	Rat
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Functional Activity
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®