PathHunter® U2OS rHTR2C β-Arrestin Cell Line

The PathHunter® U2OS rHTR2C β-Arrestin Cell Line measures rHTR2C (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells. Rat protein.

Catalog #: 93-0915C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® rHTR2C cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of rHTR2C GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged rHTR2C and EA-tagged β-Arrestin 2. Activation of rHTR2C stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rat version of this protein.
Target Class:	GPCR
Family:	5-Hydroxytryptamine Receptors (Serotonin)
Coupling:	Gq
Accession Number:	NM_012765
Target Name:	HTR2C (5-HT2C receptor)
Target Aliases:	5-hydroxytryptamine receptor 2C, 5-HT1C, 5-HT2C, 5-HTR2C, 5HTR2C, HTR1C
Target Species:	Rat
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	β-Arrestin Recruitment
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®