PathHunter® U2OS NIK Signaling Assay

The PathHunter® U2OS NIK Signaling Assay measures NFκB Signaling by activation-induced stabilization of NIK. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1059C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® NIK Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. CD40, TWEAK) induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein. Upon activation of NFκB signaling by a ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Kinase
Accession Number:	NM_003954.3
Target Name:	NIK
Target Aliases:	Mitogen-activated protein kinase kinase kinase 14
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Signaling
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®