| Biological Information | |
|---|---|
| Background Information: | The Path Hunter mFcyRIIb clustering cell line is a stable, engineered cell line designed to increase signal produced from a Checkpoint Cell Line via antibody-mediated crosslinking of the receptors. This product is a companion product used to determine cross-linking based increases in potency of agonist antibodies. The cell line included overexpresses mFcyRIIb Fcg receptor. When co-cultured with a Checkpoint Cell Line in the presence of agonist antibody, the Fcg receptor binds to the antibody, inducing receptor clustering. The induced receptor clustering leads to increased activation and downstream signaling, leading to increased EFC signal production. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. |
| Target Class: | Checkpoint Receptor |
| Family: | IgSF (Immunoglobulin Fc Receptor ) |
| Accession Number: | NM_001077189.1 |
| Target Name: | mFcyRIIb |
| Target Aliases: | Fc fragment of IgG receptor IIb |
| Target Species: | Mouse |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Stable Cell Lines |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Measures: | Clustering |
| Detection Method: | Chemiluminescence |
| Clinical Relevance | |
| Therapeutic Area: | Oncology/Immuno-Oncology |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® U2OS mFcgRIIb Clustering Cell Line
The PathHunter® U2OS mFcgRIIb Clustering Cell Line is used to determine cross-linking based potency increases of agonist antibodies. This cell line ships as two vials of cryopreserved cells. Mouse version.
User Manuals & Protocols
70-394 PathHunter Checkpoint Signaling Assay-IgSF REV3 User Manual
View DocumentDatasheets
93-1139C3
View Document