Biological Information | |
---|---|
Background Information: | The PathHunter® mCXCR4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL12/SDF-1α) based activation of mCXCR4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged mCXCR4 and EA-tagged β-Arrestin 2. Activation of mCXCR4 stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the mouse version of this protein. |
Target Class: | GPCR |
Family: | Chemokine Receptors |
Coupling: | Gi/Go |
Accession Number: | NM_009911.2 |
Target Name: | CXCR4 (SDF-1R) |
Target Aliases: | Chemokine (C-X-C motif) receptor 4, CD184, CXC-R4, CXCR-4, Cmkar4, LESTR, PB-CKR, PBSF/SDF-1, Sdf1r, b2b220Clo |
Target Species: | Mouse |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
β-Arrestin isoform: | β-Arrestin-2 |
Assay Measures: | β-Arrestin Recruitment |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS mCXCR4 β-Arrestin Cell Line
The PathHunter® U2OS mCXCR4 β-Arrestin Cell Line measures mCXCR4 (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells. Mouse protein.
User Manuals & Protocols
70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual
View DocumentDatasheets
93-0481C3 Datasheet
View Document