Biological Information | |
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Background Information: | The PathHunter® MC4R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-MSH) based activation of MC4R GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged MC4R and EA-tagged β-Arrestin 2. Activation of MC4R stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Melanocortin Receptors |
Sub Family: | Class A |
Coupling: | Gs |
Accession Number: | NM_005912.2 |
Target Name: | MC4R |
Target Aliases: | Melanocortin receptor 4, BMIQ20 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
β-Arrestin isoform: | β-Arrestin-2 |
Assay Measures: | β-Arrestin Recruitment |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS MC4R β-Arrestin Cell Line
The PathHunter® U2OS MC4R β-Arrestin Cell Line measures MC4R (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual
View DocumentDatasheets
93-0211C3 Datasheet
View Document