PathHunter® U2OS KDR Activity Assay

The PathHunter® U2OS KDR Activity Assay measures KDR receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0903C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® KDR RTK Activity cell line is a stable, engineered cell line for use in studying small molecule drug candidates targeting RTKs. This cell line enables assessment of RTK activation via detection of resulting KDR phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses a PK-tagged engineered fusion of the catalytic domain of KDR to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Kinase
Family:	Receptor Typosine Kinase (RTK): Growth Factor Receptor
Sub Family:	Protein Tyrosine
Accession Number:	NM_005228.3
Target Name:	KDR
Target Aliases:	Epidermal growth factor receptor
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Functional Activity
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®