PathHunter® U2OS IkappaB Degradation Cell Line

The PathHunter® U2OS IκB Degradation Cell Line measures pathway activation-induced degradation of IκB target protein. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0538C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® U2OS IκB Degradation Cell Line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting the NFκB pathway. This cell line enables detection of IκB levels, allowing for assessment of NFκB pathway activation-induced IκB degradation. This product uses EFC technology. The cell line included overexpresses IκB target protein fused to PL. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of IκB EFC signal is lost. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Signaling Pathway
Accession Number:	NM_020529
Target Name:	IκB
Target Aliases:	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Degradation
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®