PathHunter® U2OS HTR1B β-Arrestin Cell Line

The PathHunter® U2OS HTR1B β-Arrestin Cell Line measures HTR1B (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0697C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® HTR1B cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of HTR1B GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged HTR1B and EA-tagged β-Arrestin 2. Activation of HTR1B stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	5-Hydroxytryptamine Receptors (Serotonin)
Sub Family:	Class A
Coupling:	Gi/Go
Accession Number:	NM_000863
Target Name:	HTR1B (5-HT1B receptor)
Target Aliases:	5-hydroxytryptamine receptor 1B, 5-HT-1B, 5-HT-1D-beta, 5-HT1B, 5-HT1DB, 5-hydroxytryptamine (serotonin) receptor 1B, 5-hydroxytryptamine (serotonin) receptor 1B, G protein-coupled, 5-hydroxytryptamine receptor 1B, HTR1D2, HTR1DB, S12, serotonin 1D beta receptor, serotonin receptor 1B
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	β-Arrestin Recruitment
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®