PathHunter® U2OS HRH4 β-Arrestin Cell Line

The PathHunter® U2OS HRH4 β-Arrestin Cell Line measures HRH4 (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0209C3 Size: 2 vials

Inquire

Biological Information
Background Information:	The PathHunter® HRH4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. histamine) based activation of HRH4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged HRH4 and EA-tagged β-Arrestin 2. Activation of HRH4 stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Histamine Receptors
Sub Family:	Class A
Coupling:	Gi/Go
Accession Number:	NM_021624
Target Name:	HRH4 (H4 receptor)
Target Aliases:	Histamine receptor H4, AXOR35, BG26, GPCR105, GPRv53, H4, H4R, HH4R
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	β-Arrestin Recruitment
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®