Biological Information | |
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Background Information: | The PathHunter® GRPR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. gastrin-releasing peptide) based activation of GRPR GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged GRPR and EA-tagged β-Arrestin 2. Activation of GRPR stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Bombesin Receptor |
Sub Family: | Class A |
Coupling: | Gq |
Accession Number: | NM_005314 |
Target Name: | GRPR (BB2 receptor) |
Target Aliases: | Gastrin releasing peptide receptor, BB2, BB2R, BRS2 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
β-Arrestin isoform: | β-Arrestin-2 |
Assay Measures: | β-Arrestin Recruitment |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS GRPR β-Arrestin Cell Line
The PathHunter® U2OS GRPR β-Arrestin Cell Line measures GRPR (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual
View DocumentDatasheets
93-0229C3 Datasheet
View Document