| Biological Information | |
|---|---|
| Background Information: | The PathHunter® GHSR1a (ARR1) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. ghrelin) based activation of GHSR1a GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged GHSR1a and EA-tagged β-Arrestin-1. Activation of GHSR1a stimulates the recruitment of β-Arrestin-1 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
| Target Class: | GPCR |
| Family: | Ghrelin Receptors |
| Accession Number: | NM_198407 |
| Target Name: | GHSR1a ( Ghrelin Receptor 1a) |
| Target Aliases: | C530020I22Rik, GHRP, GHS-R1a, Ghsr |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Stable Cell Lines |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | β-Arrestin Recruitment |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® U2OS GHSR1a β-Arrestin-1 Cell Line
The PathHunter® U2OS GHSR1a β-Arrestin-1 Cell Line measures GHSR1a (GPCR) activity via recruitment of β-Arrestin-1. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual
View DocumentDatasheets
93-0242C3 Datasheet
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