Biological Information | |
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Background Information: | The PathHunter® FLT3 RTK Activity cell line is a stable, engineered cell line for use in studying small molecule drug candidates targeting RTKs. This cell line enables assessment of RTK activation via detection of resulting FLT3 phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses a PK-tagged engineered fusion of the catalytic domain of FLT3 to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Kinase |
Family: | Receptor Tyrosine Kinase |
Sub Family: | Protein Tyrosine |
Accession Number: | NM_005228.3 |
Target Name: | FLT3 |
Target Aliases: | FLT3, fms related receptor tyrosine kinase 3, FLK2, STK1, CD135, FLK-2 |
Target Species: | Human |
Cell Background: | U2OS |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Functional Activity |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | PathHunter® |
PathHunter® U2OS FLT3 Activity Assay
The PathHunter® U2OS FLT3 Activity Assay measures FLT3 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-286 PathHunter Receptor Tyrosine Kinase Activity Assay REV5 User Manual
View DocumentDatasheets
93-0901C3 Datasheet
View Document