| Biological Information | |
|---|---|
| Background Information: | The PathHunter® FGFR4-β-Klotho Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses FGF-19 induced activation of FGFR4-β-Klotho RTK receptors via detection of SH2 domain protein recruitment. The included cells overexpress ProLink-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, β-Klotho. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | Kinase |
| Family: | Receptor Typosine Kinase (RTK): Growth Factor Receptor |
| Sub Family: | Protein Tyrosine |
| Accession Number: | NM_002011 |
| Target Name: | FGFR4-α-Klotho |
| Target Aliases: | Fibroblast growth factor receptor 4, TKF, JTK2, CD334 |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Dimerization |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® U2OS FGFR4-β-Klotho Bioassay Kit
The PathHunter® FGFR4-β-Klotho Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses FGF-19 induced activation of FGFR4-β-Klotho RTK receptors via detection of SH2 domain protein recruitment
User Manuals & Protocols
70-376 PathHunter FGFR4-Beta-Klotho Bioassay Kit REV0 User Manual
View DocumentDatasheets
93-1060Y3x Datasheet
View Document