PathHunter® U2OS CXCR1 (IL8RA) β-Arrestin Cell Line

The PathHunter® U2OS CXCR1 (IL8RA) β-Arrestin Cell Line measures CXCR1 (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0226C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® CXCR1 (IL8RA) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL8/IL8) based activation of CXCR1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CXCR1 and EA-tagged β-Arrestin 2. Activation of CXCR1 stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Chemokine Receptors
Sub Family:	Class A
Coupling:	Gi/Go
Accession Number:	NM_000634
Target Name:	CXCR1 (IL8RA)
Target Aliases:	Chemokine (C-X-C motif) receptor 1, C-C, C-C-CKR-1, CD128, CD181, CDw128a, CKR-1, CMKAR1, IL8R1, IL8RA, IL8RBA, CXCR1, IL8RA, Chemokine
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
β-Arrestin isoform:	β-Arrestin-2
Assay Measures:	β-Arrestin Recruitment
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®