PathHunter® U2OS CD33 Internalization Cell Line

PathHunter® U2OS CD33 Internalization Cell Line is engineered to co-express the ProLink™ (PK) tagged CD33 receptor, and an Enzyme Acceptor (EA) tag localized to the endosomes. This stable modified cell line is provided as two vials of cryopreserved cells.

Catalog #: 93-1148C3 Size: 2 vials
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Biological Information
Background Information:
PathHunter® U2OS CD33 Internalization Cell Line is engineered to co-express the ProLink™ (PK) tagged CD33 receptor, and an Enzyme Acceptor (EA) tag localized to the endosomes. Ligand (e.g. Sialic Acid, anti-CD33 antibody) binding to the receptor stimulates receptor internalization, resulting in colocalization of the receptor-PK with the EA in the endosomes, and forcing complementation of the two β galactosidase (β-gal) enzyme fragments (EA and PK). The resulting functional β-gal enzyme hydrolyzes substrate to generate a chemiluminescent signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Checkpoint Receptor
Family:
T cell Receptor (inhibitory)
Sub Family:
Protein Tyrosine
Accession Number:
NM_001772.3
Target Name:
CD33 (SIGLEC3)
Target Aliases:
Myeloid cell surface antigen CD33
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Internalization
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-413 PathHunter Internalization Assays User Manual REV0

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Datasheets

93-1148C3

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